The Evolution of Microbial
Life Chapter 15.
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1. Major Episodes in the History of Life. See
figure 15.2
- Give the approximate time bp(before the present) for the
following events:
- Origin of the Earth
- Oldest prokaryote fossils
- Accumulation of oxygen
- Oldest eukaryote fossils
- Oldest animal fossils
- Give the approximate time and significance of the Cambrian
explosion.
2. The Origin of Life
- Explain what is meant by the biogenesis paradox
- List and discuss the four steps hypothesized in your text for the origin
of life:
- Stage 1. Abiotic synthesis of organic monomers
- Explain why Stanley Miller's experiment was significant
- List the other lines of evidence for the easy synthesis of organic monomers
under abiotic conditions
- Stage 2. Synthesis of organic polymers.
- Stage 3. Synthesis of Self replicating systems.
- Explain the experimental evidence that perhaps RNA was first genetic
material.
- Define ribozymes and explain their significance in understanding how
life might have arisen.
- Stage 4. Formation of pre-cells
Comment: This material is
highly speculative and we may never understand how the first cells
arose. But these sorts of steps seem reasonable based on what we know
today about chemistry. Also they Stage 3 and 4 may have happened in
parallel. For instance small random polypeptides and RNA molecules show
the ability to catalyze chemical reactions and some scientists have
proposed that sets of these molecules, able to catalyze their own
synthesis may have been an important first step. Some scientist even
propose that the first replicating pre-cells may not have involved any
sort of genetic material at all! The formation of pre cells involves
the idea that certain common types of organic molecules such as
phospholipids and proteins spontaneously self assemble into more
complex structures. See figs 15.7. A good recent overview about self
assembly is http://www2.bc.edu/~strother/GE_146/lectures/14.html
as well as this talk ( http://www.theharbinger.org/articles/rel_sci/fox.html
) given at the Vatican by one of the major investigators in this area, Sidney
Fox. Self assembly is being exploited in the developing field of nanotechnology.
See http://physicsweb.org/articles/news/8/6/7/1
But also see this highly skeptical paper: http://origins.swau.edu/papers/life/chadwick/default.html
(I think the logic is flawed but worth looking at)
Here is a more balanced yet skeptical viewpoint: http://www.nytimes.com/library/national/science/040699sci-cell-rna.html
My own view point has not changed much since I wrote this essay in
1999: http://staff.jccc.net/pdecell/essays/notall.htm
3. Prokaryotes
- List the major roles of prokaryotes in the environment.
- Distinguish between the Bacteria and the Archaea
- Define extremophiles, halophiles, thermophiles and methanogens
- Review the general structure of prokaryotic cells. Fig 4.5
- Distinguish between the main shapes of prokaryotes and give an example of
a bacteria with each shape:
- Cocci
- Bacilli
- Spirochetes
- Describe the structure of Bacterial flagella and explain how these flagella
are different from eukaryotic flagella
- Define binary fission and explain how this process leads to explosive population
growth in bacteria.
- Explain how bacteria are able to survive harsh environments. p 306
- Discuss the nutritional diversity of bacteria
- List and define the main modes of nutrition in bacteria and in other
organisms:
- photoautotrophs
- chemoautotrophs
- photoheterotrophs
- chemoheterotrophs
- Discuss the ecological impact of bacteria
- Define the term pathogen
- Distinguish between exotoxins and endotoxins.
- Explain the importance of bacteria in chemical recycling and give examples.
- Define bioremediation how bacteria can be used for bioremediation.
The Bacterial Flagellum and "Intelligent
Design":
The bacterial flagellum is often held up as a possible example of Intelligent
design. Since it is composed of lots of different proteins including a rotating
wheel it seems that it could not have evolved through a series of transition.
The main advocate of this position is Michael Behe, for example on this intelligent
design site.
http://www.arn.org/docs/mm/flagellum_all.htm
But .....check out this site and rebuttal to Behe by Ian Musgrave:
http://www.health.adelaide.edu.au/Pharm/Musgrave/essays/flagella.htm
You read..you decide. :-)
Also here is a really cool site that discusses how the Bacterial Flagellum
self assembles!
http://www.aip.org/png/2002/174.htm
A more general rebuttal to intelligent design arguements with special focus
on Bacterial Flagella is http://www.millerandlevine.com/km/evol/design2/article.html
4. Protists
- Explain the two processes believed responsible for the evolution
of eukaryotes
- Define symbiosis and explain the role that symbiosis is believed
to have played in the evolution of eukaryotes
- Discuss the evidence for Lynn Margulis's endosymbiotic hypothesis.
A good discussion of Dr. Margulis's work is http://www.nyu.edu/classes/neimark/margulis.html
Also check out this recent article:
http://edition.cnn.com/2005/TECH/science/10/14/new.organism.reut/
Comment: Today some scientists suggest that the original function of mitochondria
was not to produce ATP but to detoxify oxygen. Only later according to this
hypothesis did the electron transport systems in the mitochondria become important
for ATP production. If true, then this would be an example of exaptation
as defined in chapter 14.
5. Diversity of protists.
- List and give examples of the following groups of "protozoans"
- Flagellates
- Amoebas
- Apicomplexans
- Ciliates
- Distinguish between the two types of Slime "molds"
- Plasmodial slime molds
- Cellular slimes molds
- Distinguish between the main groups of unicellular algae.
- Dinoflagellates
- Diatoms
- Green algae
- Define and give examples of "seaweed"
You might enjoy visiting my Protista pages at http://staff.jccc.net/pdecell/protista/protistframe.html
A much more comprehensive and interactive Protist site is at: http://www.sidwell.edu/us/science/vlb5/Labs/Classification_Lab/Eukarya/Protista/
Comment: The Kingdom Protista is structurally
the most diverse of the currently recognized kingdoms. It is likely that this
Kingdom will get broken up as we infer more about their evolutionary relationships.
For instance the 'green algae' are quite different from each other and probably
do not have an immediate common ancestor. Some of the multicellular algae are
more like plants and probably belong in the plant kingdom. Chloroplasts in the
protista are highly diverse and suggest several independent origins for photosynthesis
in eukaryotes. Even the eukaryotes as a group may not have arisen from one type
of prokaryote. For instance, the cell membrane of eukaryotes tends to be similar
to that of bacteria but the DNA and ribosomes appear more similar to the Archaea,
suggesting that the common ancestor of eukaryotes might have arisen from an
ancestor that itself was an amalgam of bacteria and archaean cells! The biochemistry
of prokaryotes and protists probably holds many suprizes for us!
6. Viruses: Genes in packages. Chapter 10 pp 188-189
- Explain the main features of viruses that are similar to living things
- Explain how viruses differ from living things.
- Explain the typical structure of bacteriophages
- Distinugish between the lytic and lysogenic virus reproductive cycle.
- Describe the structure of the tobacco mosaic virus as a representative plant
virus.
- Describe the basic structure of the influenza virus as a representative
animal virus.
- Define retroviruses as distinct from other types of RNA viruses.
- Describe the reproductive cycle of the HIV virus as a representative retrovirus.
- Explain the role of reverse transcriptase in the reproductive cycle of theHIV
virus
- Explain the term 'emerging virus' and give several examples of emerging
viruses.
pgd created 11/20/04 revised 04/08/06